Convergence properties of solitary tract neurons responsive to cardiac receptor stimulation in the anesthetized cat

The convergence pattern of cardiac receptors, pulmonary C-fibers, carotid chemoreceptor, and baroreceptor afferents onto neurons within the nucleus of the solitary tract (NTS) was studied in the anesthetized (pentobarbitone sodium, 40 mg/kg,) paralyzed and artificially ventilated cat. Extra- and intracellular recordings were made from NTS neurons while stimulating both cardiac receptors by aortic root injections of veratridine (1-3 micrograms/kg) and pulmonary C-fibers by a right atrial injection of phenylbiguanide (10-20 micrograms/kg). The ipsilateral carotid body was stimulated by using arterial injection of CO2-saturated bicarbonate solution, whereas inflation of the ipsilateral carotid sinus was used to activate baroreceptors. The ipsilateral cardiac vagal branch, cervical vagus, and carotid sinus nerves were stimulated electrically (1 Hz, 0.2-1 ms, 1-35 V). In 78 NTS neurons recorded either extracellularly (n = 47) or intracellularly (n = 31), electrical stimulation of the cardiac branch of the vagus nerve evoked synaptic potentials (spikes and/or excitatory postsynaptic potentials) with an onset latency between 4 and 220 ms. Some neurons displayed both short and long latency inputs(15.5 +/- 1.8 and 160.0 +/- 8.5 ms; n = 14). Of these 78 neurons, 24 responded to veratridine stimulation of cardiac receptors (i.e., cardioreceptive neurons) by exhibiting an augmenting-decrementing discharge of 37 +/- 4 s in duration with a peak frequency of 30 +/- 5 Hz. Convergence from other cardiorespiratory receptors was noted involving either carotid chemoreceptors (n = 7) or pulmonary C-fibers (n = 4) or from both carotid chemoreceptors and pulmonary C-fibers (n = 6). In contrast, only one cardioreceptive NTS neuron was activated by distension of the carotid sinus. Recording sites recovered were confined to the medial NTS at the level of the area postrema and extended caudally into the commissural subnucleus. Our results indicate a convergence of carotid chemoreceptor and pulmonary C-fiber afferent inputs to cardioreceptive NTS neurons. With the paucity of baroreceptor inputs to these neurons it is suggested that sensory integration within the NTS may reflect regulatory versus defensive or protective reflex control.     

Apolipoprotein E*3-Leiden transgenic mice as a test model for hypolipidaemic drugs

PURPOSE: We report an investigation into the distribution of proteoglycans (PGs) in normal, organ-cultured and dextran-treated human corneas. METHODS: Immunogold labeling was carried out at the electron microscope level to localize keratan sulphate (KS), chondroitin sulphate (CS), and heparan sulphate (HS) PGs. RESULTS: High levels of labeling for CS was found in the epithelium, endothelium, and keratocytes, with light labelling present in the basement membranes and the corneal stroma. Labeling for HS was present in the epithelium, endothelium, and keratocytes, with intense labeling present at the endothelium/Descemet's membrane interface and the epithelium/Bowman's layer interface. Large filaments were also observed in these regions in cuprolinic blue-stained specimens. Keratan sulphate was present at high levels in the stroma and the basement membranes with low levels present within the keratocytes, epithelium, and endothelium. The pattern of KS labeling along the collagen fibrils in the stroma sometimes showed evidence of periodicity. Organ-cultured corneas had extensive collagen-free "lakes," the interior of which immunolabeled positively for KS and showed staining with cuprolinic blue. The lakes were greatly reduced in the dextran-treated samples. CONCLUSION: This investigation determined the ultrastructural distribution of KS, CS, and HS PGs in human cornea and showed that organ culture is associated with a change in distribution of stromal PGs.     

Basic trends of scientific research into the problem of titanium in the Ukraine

We analyze the state-of-the-art and prospects of the research into the development of physical and metallurgical foundations of creation of new alloys based on titanium and cost-efficient technologies of their production and treatment at the Ukrainian scientific-research institutions with an aim to improve their service properties and, hence, promote more extensive application of these unique structural materials. __________ Translated from Fizyko-Khimichna Mekhanika Materialiv, Vol. 42, No. 3, pp. 5–16, May–June, 2006.     

User interface modeling in UMLi

Although user interfaces represent an essential part of software systems, the Unified Modeling Language )UML) seems to have been developed with little specific attention given to user interface issues. Several researchers have investigated integrating interface modeling techniques with UML. In UML, one models tasks using extended activity diagrams rather than by incorporating a completely new notation into UML. UMLi also addresses the relationships between use cases, tasks, and views, and thoroughly addresses the relationship between tasks and the data on which they act. UMLi is probably the most technically mature proposal for interface development in UML.     

QoS-aware optimization of sensor network queries

The resource-constrained nature of mote-level wireless sensor networks (WSNs) poses challenges for the design of a general-purpose sensor network query processors (SNQPs). Existing SNQPs tend to generate query execution plans (QEPs) that are selected on the basis of a fixed, implicit expectation, for example, that energy consumption should be kept as small as possible. However, in WSN applications, the same query may be subject to several, possibly conflicting, quality-of-service (QoS) expectations concomitantly (for example maximizing data acquisition rates subject to keeping energy consumption low). It is also not uncommon for the QoS expectations to change over the lifetime of a deployment (for example from low to high data acquisition rates). This paper describes optimization algorithms that respond to stated QoS expectations (about acquisition rate, delivery time, energy consumption and lifetime) when making routing, placement, and timing decisions for in-WSN query processing. The paper shows experimentally that QoS-awareness offers significant benefits in responding to, and reconciling, diverse QoS expectations, thereby enabling QoS-aware SNQPs to generate efficient QEPs for a broader range WSN applications than has hitherto been possible.     

Simulated Bacterially-Inspired Problem Solving – The Behavioural Domain

Bacterial populations meet the challenges of dynamic spatially heterogeneous environments with fluctuating biotic and abiotic factors in a number of ways. The motivation for the work presented here has been to transfer ideas from bacterial adaptability and evolvability to computational problem solving. Following a brief comment on some examples of the ways bacteria solve problems, a bacterially-inspired computational architecture for simulating aspects of problem solving is described. We then examine three case studies. The first, a study of the mutational impact of a remediation to toxic (fitness-reducing) material, highlights how a sufficiently pre-engineered adaptive system can solve a difficult problem quite easily. The second study looks at why it is difficult to evolve complex problem solving behaviours and how artificial selection mechanisms coupled with pre-engineering the system can help. Specifically, this refers to quorum sensing and tactic behaviours. A further study looked at ways in which a quorum sensing analogue could help computational agents find multiple peaks in a landscape. The paper concludes with a discussion of an investigation of bacteria that had both quorum sensing and tactic capabilities.